The Phospho-HSPB1 (S15) Polyclonal Antibody (CABP0040) is a valuable tool for researchers studying HSPB1, a heat shock protein involved in cellular stress response and protection. This antibody, generated in rabbits, is highly specific to the phosphorylated form of HSPB1 at serine 15 and is validated for use in Western blot applications. By detecting and binding to phosphorylated HSPB1, researchers can investigate its role in cellular signaling pathways and stress response mechanisms.HSPB1, also known as heat shock protein 27 (Hsp27), is a key player in protecting cells from stress-induced damage, making it a potential therapeutic target for diseases characterized by cellular stress, such as neurodegenerative disorders and cardiovascular diseases.
Understanding the function and regulation of phospho-HSPB1 (S15) is essential for developing strategies to enhance cellular stress resistance and prevent stress-related diseases.Overall, the Phospho-HSPB1 (S15) Polyclonal Antibody (CABP0040) is a valuable tool for researchers interested in studying the role of HSPB1 in cellular stress response and its potential implications in diseases related to cellular stress. Its specificity to the phosphorylated form of HSPB1 at serine 15 makes it a reliable and effective tool for investigating the function of this important heat shock protein.
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Purification Method:
Affinity purification
Gene ID:
3315
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-HSP27/HSPB1-S15 Rabbit pAb (CABP0040) at 1:600 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
