The Phospho-Hsp27 (S82) Rabbit Monoclonal Antibody (CABP1031) is a valuable tool for researchers studying the phosphorylation of Hsp27 at Serine 82, a critical post-translational modification involved in cellular stress response and signaling pathways. This antibody, produced in rabbits, exhibits high specificity and sensitivity in detecting phosphorylated Hsp27 (S82) in human samples, and has been validated for use in Western blot applications.Hsp27, also known as heat shock protein 27, is a chaperone protein that plays a crucial role in protecting cells from stress-induced damage and promoting cell survival.
Phosphorylation of Hsp27 at Serine 82 is known to regulate its function in response to various stress stimuli, making it a key target for investigation in cancer research, neurodegenerative diseases, and other pathological conditions.By enabling the precise detection and analysis of phosphorylated Hsp27 (S82) in different cell types, the CABP1031 antibody offers researchers a powerful tool for studying the molecular mechanisms underlying cellular stress responses and identifying potential therapeutic targets for diseases associated with dysregulated stress signaling pathways.
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Purification Method:
Affinity purification
Gene ID:
3315
Clone Number:
ARC1576
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-HSP27/HSPB1-S82 Rabbit mAb (CABP1031) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
