The Phospho-Hsp27 (S78) Rabbit Monoclonal Antibody (CABP1032) is a powerful tool for researchers studying the phosphorylation of Hsp27 at serine 78, a key post-translational modification linked to stress response and cell survival. This antibody, generated from rabbit monoclonal cells, exhibits high specificity and sensitivity for detecting phosphorylated Hsp27 in human samples, making it an essential tool for investigating cellular stress pathways.Phosphorylation of Hsp27 at serine 78 is known to regulate the chaperone activity of Hsp27, thereby influencing cellular responses to stressors such as heat shock, oxidative stress, and chemotherapy. By targeting this specific phosphorylation site, researchers can gain valuable insights into the mechanisms underlying stress adaptation and cell survival, with potential implications for cancer biology, neurodegenerative disorders, and other diseases characterized by cellular stress.
With its superior performance in applications such as Western blotting, immunofluorescence, and immunoprecipitation, the Phospho-Hsp27 (S78) Rabbit Monoclonal Antibody (CABP1032) offers a reliable means of studying phosphorylated Hsp27 dynamics in various cell types and experimental conditions. By utilizing this antibody, researchers can uncover new insights into the role of Hsp27 phosphorylation in cell signaling pathways and disease progression, paving the way for innovative therapeutic strategies targeting stress response pathways.
This gene encodes a member of the small heat shock protein (HSP20) family of proteins. In response to environmental stress, the encoded protein translocates from the cytoplasm to the nucleus and functions as a molecular chaperone that promotes the correct folding of other proteins. This protein plays an important role in the differentiation of a wide variety of cell types. Expression of this gene is correlated with poor clinical outcome in multiple human cancers, and the encoded protein may promote cancer cell proliferation and metastasis, while protecting cancer cells from apoptosis. Mutations in this gene have been identified in human patients with Charcot-Marie-Tooth disease and distal hereditary motor neuropathy.
Purification Method:
Affinity purification
Gene ID:
3315
Clone Number:
ARC1577
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Phospho-HSP27/HSPB1-S78 Rabbit mAb (CABP1032) at 1:1000 dilution.HeLa cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3s.
