The Phospho-HSL (S660) Monoclonal Antibody is a highly specific tool designed for research involving HSL (Hormone sensitive lipase) phosphorylated at serine 660. HSL is a key enzyme involved in the regulation of lipid metabolism and plays a crucial role in the mobilization of stored fats in the body.This monoclonal antibody, produced through advanced technology, is highly reactive with phosphorylated HSL at serine 660 in human, mouse, and rat samples. It is validated for use in various applications including Western blot, immunohistochemistry, and immunofluorescence, allowing for precise detection and analysis of phospho-HSL (S660) in different cell types and tissues.
Understanding the phosphorylation status of HSL at serine 660 is essential for unraveling the complex mechanisms underlying lipid metabolism, energy balance, and metabolic disorders such as obesity, diabetes, and cardiovascular diseases. This antibody serves as a valuable tool for researchers in the fields of metabolism, endocrinology, and obesity-related research, providing insights into the regulation of lipid breakdown and potential therapeutic targets for metabolic disorders.
Product Name:
Phospho-HSL-S660 Monoclonal Antibody
SKU:
CABP1432
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthesized peptide derived from human HSL (Phospho-Ser660)
The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.
Purification Method:
Affinity purification
Gene ID:
3991
Clone Number:
ARC61254
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of 3T3-L1 cells, using Phospho-HSL-S660 Rabbit mAb (CABP1432) at 1:1000 dilution.3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0.06mol/L alcohol and treated with isoproterenol (10 μM, 20 min).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
