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Phospho-HSL-S660 Monoclonal Antibody (CABP1432)

SKU:
CABP1432
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-HSL-S660 Monoclonal Antibody
SKU:CABP1432
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Mouse
Immunogen:A synthesized peptide derived from human HSL (Phospho-Ser660)
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:HSL; LHS; REH; AOMS4; FPLD6; Phospho-HSL-S660
Positive Sample:3T3-L1
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Membrane, caveola, cytosol.
Calculated MW:117kDa
Observed MW:84kDa

The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids.

Purification Method:Affinity purification
Gene ID:3991
Clone Number:ARC61254
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of 3T3-L1 cells, using Phospho-HSL-S660 Rabbit mAb (CABP1432) at 1:1000 dilution.3T3-L1 cells that were not differentiated or differentiated into adipocytes treated with 0.06mol/L alcohol and treated with isoproterenol (10 μM, 20 min).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.

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