The Phospho-HSF1 (S326) Rabbit Monoclonal Antibody (CABP1140) is a valuable tool for researchers studying heat shock factor 1 (HSF1) phosphorylation at serine 326, a critical post-translational modification that regulates HSF1 activity. This antibody, specifically designed for use in Western blot applications, is highly sensitive and specific for detecting phosphorylated HSF1 at S326 in human samples.HSF1 is a key transcription factor that plays a central role in cellular stress responses, particularly in regulating the expression of heat shock proteins that protect cells from proteotoxic stress. Phosphorylation of HSF1 at S326 has been shown to modulate its transcriptional activity, impacting cellular responses to stress conditions such as heat shock and oxidative stress.
By using the Phospho-HSF1 (S326) Rabbit Monoclonal Antibody, researchers can accurately assess the phosphorylation status of HSF1 at S326 in various experimental settings, providing insights into the molecular mechanisms underlying stress-induced signaling pathways. This antibody is a valuable tool for studies investigating the role of HSF1 phosphorylation in cellular stress responses, aging, neurodegenerative diseases, and cancer, and may lead to the identification of novel therapeutic targets for these conditions.
Product Name:
Phospho-HSF1-S326 Rabbit Monoclonal Antibody
SKU:
CABP1140
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S326 of human HSF1 (Q00613).
Sequence:
LLSP T
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
HSTF1; Phospho-HSF1-S326
Positive Sample:
293T treated by PMA/TPA
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
82kDa
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Purification Method:
Affinity purification
Gene ID:
3297
Clone Number:
ARC1601
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-HSF1-S326 Rabbit mAb (CABP1140) at 1:1000 dilution.293T cells were treated by PMA/TPA (200 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1min.
