The Phospho-HSF1 (S307) Polyclonal Antibody is a valuable tool for researchers studying the heat shock protein HSF1, specifically phosphorylated at serine 307. This antibody, raised in rabbits, is highly specific for human samples and is validated for use in Western blot applications. By binding to the phosphorylated form of HSF1, researchers can detect and analyze its activity in various cellular contexts.HSF1, a key regulator of the heat shock response, plays a crucial role in proteostasis and cellular stress response. Phosphorylation at serine 307 is known to modulate HSF1 activity, influencing its ability to regulate the transcription of heat shock proteins.
Understanding the phosphorylation status of HSF1 can provide insights into how cells respond to stress and maintain proteostasis.Research into the phosphorylation of HSF1 at serine 307 is essential for understanding the mechanisms of cellular stress response and may have implications for diseases related to protein misfolding and proteotoxicity. This antibody is a valuable tool for studying HSF1 function and may contribute to the development of novel therapeutic strategies targeting the heat shock response pathway.
Product Name:
Phospho-HSF1-S307 Polyclonal Antibody
SKU:
CABP1396
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S307 of human HSF1.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
HSTF1; Phospho-HSF1-S307
Positive Sample:
HUVEC
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
57kDa
Observed MW:
76kDa
The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90.
Purification Method:
Affinity purification
Gene ID:
3297
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-HSF1-S307 antibody (CABP1396) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
