The Phospho-Histone Hist1H3a (T3) Polyclonal Antibody (CABP0846) is a valuable tool for researchers studying histone modifications and their role in gene expression regulation. This antibody, raised in rabbits, specifically targets the phosphorylated form of Histone Hist1H3a at threonine 3 (T3) and is highly reactive with human samples. Validated for use in Western blot applications, it allows for the detection and analysis of histone modification in various cell types.Histone modifications, such as phosphorylation, play a critical role in chromatin remodeling and gene transcription regulation. The phosphorylation of Histone Hist1H3a at threonine 3 (T3) in particular has been implicated in processes like DNA repair, cell cycle progression, and apoptosis.
Studying this specific histone modification can provide valuable insights into various biological processes and diseases, including cancer and developmental disorders.By using the Phospho-Histone Hist1H3a (T3) Polyclonal Antibody (CABP0846), researchers can delve deeper into the epigenetic mechanisms underlying gene expression regulation and potentially identify new therapeutic targets for disease intervention. Its high specificity and reactivity make it an essential tool for studies in epigenetics, cancer biology, and other fields related to histone modification research.
Product Name:
Phospho-Histone H3-T3 Rabbit Polyclonal Antibody
SKU:
CABP0846
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Histone H3-T3 pAb (CABP0846) at 1:1000 dilution.C6 and HeLa cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.C6 and NIH/3T3 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
