Phospho-Histone H3 (Thr11) Antibody (PACO23916)
- SKU:
- PACO23916
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Phospho-Histone H3 (Thr11) Antibody (PACO23916)
The Phospho-Histone H3 (Thr11) Antibody (PACO23916) is a valuable tool for researchers studying cell division and chromatin structure. This antibody, raised in rabbits, is highly specific and sensitive for detecting phosphorylation of Histone H3 at Threonine 11 in human samples. It has been validated for use in Western blot and immunofluorescence applications, allowing researchers to visualize and analyze this important modification in various cell types.Histone H3 phosphorylation at Thr11 is a key event during mitosis, playing a crucial role in chromosome condensation and segregation. Dysregulation of this phosphorylation event has been associated with various diseases, including cancer, making it an attractive target for research in oncology and cell biology.
By using the Phospho-Histone H3 (Thr11) Antibody, researchers can gain valuable insights into the mechanisms underlying cell division and potentially uncover new therapeutic targets for cancer treatment.Overall, the Phospho-Histone H3 (Thr11) Antibody is a valuable tool for researchers interested in studying cell division, chromatin dynamics, and disease mechanisms. Its high specificity and sensitivity make it a reliable reagent for detecting Histone H3 phosphorylation at Thr11, opening up new avenues for exploration in the fields of cancer biology and cell cycle regulation.
| Antibody Name: | Phospho-Histone H3 (Thr11) Antibody (PACO23916) |
| Antibody SKU: | PACO23916 |
| Size: | 100ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:500-1:1000 |
| Species Reactivity: | Human, Mouse, Rat |
| Immunogen: | Peptide sequence around phosphorylation site of threonine 11(K-S-T(p)-G-G) derived from Human Histone H3. |
| Form: | Liquid |
| Storage Buffer: | Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Purification Method: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western blot analysis of extracts from U87 cells untreated or treated with EGF using Histone H3(Phospho-Thr11) Antibody. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Synonyms: | H3/a H3/m H3.3A; H3/c H3/o H3F3B; H3/d; H3/f; H3/h |
| UniProt Protein Function: | H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P68431 |
| NCBI GenInfo Identifier: | 55977055 |
| NCBI Gene ID: | 8357 |
| NCBI Accession: | P68431.2 |
| UniProt Secondary Accession: | P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1, |
| UniProt Related Accession: | P68431 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | Histone H3.1 |
| NCBI Synonym Full Names: | histone cluster 1 H3 family member h |
| NCBI Official Symbol: | HIST1H3HÂ Â |
| NCBI Official Synonym Symbols: | H3/k; H3FK; H3F1KÂ Â |
| NCBI Protein Information: | histone H3.1 |
| UniProt Protein Name: | Histone H3.1 |
| UniProt Synonym Protein Names: | Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
| UniProt Gene Name: | HIST1H3AÂ Â |
| Secondary Antibody |
| Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014) |
| Recommended Products |
| Anti-FITC Goat Anti-Rabbit IgG (H+L) Antibody (CABS011) |
| Anti-HRP-conjugated Beta Actin Antibody (CABC028) |
Related Products
Phospho-Histone H3 (Thr11) Antibody (PACO00119)
Phospho-Histone H3 (Thr6) Antibody (PACO00117)
Phospho-Histone H3 (S10) Antibody (PACO06128)
Phospho-Histone H3 (Thr45) Antibody (PACO00123)
Phospho-Histone H3 (T11) Antibody (PACO05460)
Phospho-Histone H3 (S28) Antibody (PACO06129)
Phospho-Histone H3 (Thr32) Antibody (PACO00121)
Phospho-Histone H3 (Thr3) Antibody (PACO00116)
