The Phospho-Histone H3 (T6) Rabbit Polyclonal Antibody (CABP0899) is a valuable tool for researchers studying histone H3, a protein central to the regulation of gene expression through its role in chromatin structure. This antibody, developed using rabbits as the host organism, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the phosphorylated form of Histone H3 at threonine 6, this antibody enables precise detection and analysis of this critical histone modification in various cell types.The phosphorylation of Histone H3 at threonine 6 is known to play a role in regulating chromatin dynamics and gene transcription, making it a key player in cellular processes such as cell division, DNA repair, and apoptosis.
Research into the phosphorylation status of Histone H3 can provide valuable insights into cellular signaling pathways and epigenetic mechanisms that govern gene expression. This antibody is an essential tool for scientists studying epigenetics, cancer biology, and developmental biology, as well as those exploring potential therapeutic targets in various disease contexts.
Product Name:
Phospho-Histone H3-T6 Rabbit Polyclonal Antibody
SKU:
CABP0899
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around T6 of human (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C2C12 cells, using Phospho-Histone H3-T6 Rabbit pAb (CABP0899) at 1:400 dilution.C2C12 cells were treated by nocodazole (50 ng/ml) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
