The Phospho-Histone H3 (T45) Rabbit Polyclonal Antibody (CABP0898) is a valuable tool for researchers studying histone modifications and their impact on cellular processes. Histone H3 is a core component of nucleosomes, the structural units of chromatin, and the phosphorylation of specific residues, such as threonine 45, plays a key role in regulation of gene expression and cell division.This antibody, produced in rabbits, is highly specific for detecting phosphorylated histone H3 at threonine 45 in human samples. It has been validated for use in Western blot applications, allowing for precise and sensitive detection of this specific histone modification.
By targeting phospho-histone H3 (T45), researchers can gain valuable insights into the regulation of chromatin structure and the control of cell cycle progression.Studies have shown that aberrant phosphorylation of histone H3 at threonine 45 is associated with various diseases, including cancer. By using the Phospho-Histone H3 (T45) Rabbit Polyclonal Antibody, researchers can further investigate the role of this histone modification in disease development and progression. This antibody is an essential tool for researchers working in the fields of epigenetics, cancer biology, and cell cycle regulation.
Product Name:
Phospho-Histone H3-T45 Rabbit Polyclonal Antibody
SKU:
CABP0898
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around T45 of human Histone H3 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Histone H3-T45 Rabbit pAb (CABP0898) at 1:1000 dilution. Hela cells, NIH/3T3 cells and C6 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour or treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
