The Phospho-Histone H3 (T3) Rabbit Monoclonal Antibody (CABP1152) is a powerful tool for researchers studying cell division and chromatin structure. This antibody specifically targets phosphorylated Threonine 3 on Histone H3, a key event in the condensation of chromosomes during mitosis. Raised in rabbits, this antibody is highly sensitive and selective for detecting Histone H3 phosphorylation at T3, making it ideal for use in applications such as immunofluorescence and chromatin immunoprecipitation. Its high specificity allows for precise visualization and measurement of Histone H3 phosphorylation, providing valuable insights into the regulation of cell division and gene expression.
With its ability to target a critical marker of chromatin dynamics during cell division, the Phospho-Histone H3 (T3) Rabbit Monoclonal Antibody (CABP1152) is an essential tool for researchers studying cell biology, cancer biology, and epigenetics. Its reliable performance and accurate results make it a valuable asset in understanding the intricate mechanisms that govern cell cycle progression and gene regulation.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Clone Number:
ARC1662
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H3.3-T3 Rabbit mAb (CABP1152) at 1:1000 dilution.NIH/3T3 cells were treated by Nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 3min.
