The Phospho-Histone H3 (T11) Polyclonal Antibody (CABP0093) is a valuable tool for researchers studying phosphorylation events in histone proteins. Histone H3 is a crucial component of chromatin structure and plays a role in gene expression regulation, DNA repair, and cell division.This antibody, raised in rabbits, is highly specific for phosphorylated Threonine 11 of Histone H3 and is validated for use in various applications, including Western blot and immunohistochemistry. It enables precise detection and quantification of phosphorylated Histone H3 in samples from various organisms, making it ideal for studies in epigenetics, cell biology, and cancer research.
Phosphorylation of Histone H3 at Threonine 11 is associated with different cellular processes, such as mitosis, DNA damage response, and gene transcription. Understanding the role of this post-translational modification is crucial for unraveling the mechanisms underlying various biological processes and diseases. The Phospho-Histone H3 (T11) Polyclonal Antibody is a valuable tool for researchers investigating these complex pathways and their implications in health and disease.
Product Name:
Phospho-Histone H3-T11 Rabbit Polyclonal Antibody
SKU:
CABP0093
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around T11 of human HIST1H3B (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-Histone H3-T11 Rabbit pAb (CABP0093) at 1:2000 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
