The Phospho-Histone H3 (S28) Polyclonal Antibody (CABP0839) is a valuable tool for researchers studying histone modifications and cell cycle regulation. This antibody, produced in rabbits, is highly specific for detecting phosphorylation of Histone H3 at Serine 28 in human samples. Validated for use in Western blotting applications, this antibody allows for the precise detection and analysis of phosphorylated Histone H3 in various cell types.Histone H3 is a core histone protein that plays a crucial role in chromatin structure and gene regulation. Phosphorylation of Histone H3 at Serine 28 is known to be involved in mitotic chromosome condensation and cell cycle progression, making it a key marker for studying cell division and proliferation processes.
By targeting this specific phosphorylation site, researchers can gain invaluable insights into the mechanisms controlling cell cycle progression and chromatin remodeling.The Phospho-Histone H3 (S28) Polyclonal Antibody is an essential tool for studies in molecular biology, cell biology, and cancer research. Its ability to specifically detect phosphorylated Histone H3 at Serine 28 makes it a valuable asset for researchers investigating the intricate mechanisms underlying cell cycle regulation and chromatin dynamics. By utilizing this antibody, researchers can further our understanding of cell division processes and potentially identify novel therapeutic targets for diseases characterized by dysregulated cell proliferation.
Product Name:
Phospho-Histone H3-S28 Rabbit Polyclonal Antibody
SKU:
CABP0839
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic phosphorylated peptide around S28 of human Histone H3 (NP_003520.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-Histone H3-S28 Rabbit pAb (CABP0839) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
