Anti-Phospho-Histone H3-S10/T11 Antibody (CABP0896)
- SKU:
- CABP0896
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-Phospho-Histone H3-S10/T11 Antibody (CABP0896)
The Phospho-Histone H3 (S10/T11) Rabbit Polyclonal Antibody (CABP0896) is a valuable tool for researchers studying histone modifications and cell cycle regulation. This antibody specifically recognizes the phosphorylated form of histone H3 at serine 10 and threonine 11, critical sites involved in chromatin condensation and DNA replication during mitosis.Raised in rabbits, this antibody is highly reactive with human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of histone H3, researchers can investigate the dynamics of chromatin structure and gene expression during cell division, providing insights into cell proliferation, differentiation, and tumor development.
Histone modifications, such as phosphorylation of histone H3, play a key role in regulating gene expression and chromatin remodeling. The phosphorylation of histone H3 at serine 10 and threonine 11 is associated with chromosome condensation and segregation during mitosis, making this antibody a valuable tool for studying cell cycle progression and mitotic events.In summary, the Phospho-Histone H3 (S10/T11) Rabbit Polyclonal Antibody (CABP0896) is a reliable reagent for researchers investigating histone modifications, cell cycle regulation, and chromatin dynamics. Its specificity and sensitivity make it an essential tool for studies in cell biology, cancer research, and epigenetics.
| Antibody Name: | Anti-Phospho-Histone H3-S10/T11 Antibody |
| Antibody SKU: | CABP0896 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Host Species: | Rabbit |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S10/T11 of human Histone H3. |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat, Other (Wide Range) |
| Positive Samples: | HeLa, NIH/3T3, C6 |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S10/T11 of human Histone H3. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 8350 |
| Uniprot: | P68431 |
| Cellular Location: | Chromosome, Nucleus |
| Calculated MW: | 15kDa |
| Observed MW: | 17kDa |
 | Western blot - Phospho-Histone H3-S10/T11 Rabbit pAb (CABP0896) |
| Synonyms: | H3/A, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3C10, H3C11, H3C12, HIST1H3A |
| Background: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Protein Function: | H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P68431 |
| NCBI GenInfo Identifier: | 55977055 |
| NCBI Gene ID: | 8357 |
| NCBI Accession: | P68431.2 |
| UniProt Secondary Accession: | P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1, |
| UniProt Related Accession: | P68431 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | Histone H3.1 |
| NCBI Synonym Full Names: | histone cluster 1 H3 family member h |
| NCBI Official Symbol: | HIST1H3HÂ Â |
| NCBI Official Synonym Symbols: | H3/k; H3FK; H3F1KÂ Â |
| NCBI Protein Information: | histone H3.1 |
| UniProt Protein Name: | Histone H3.1 |
| UniProt Synonym Protein Names: | Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
| UniProt Gene Name: | HIST1H3AÂ Â |
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