The Phospho-Histone H2B (Ser14) Antibody (PACO00172) is a valuable tool for researchers studying post-translational modifications of histones, specifically phosphorylation of Histone H2B at Serine 14. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in various research applications such as Western blotting.Histone phosphorylation plays a crucial role in the regulation of gene expression, chromatin structure, and cellular processes such as mitosis and DNA repair. Phosphorylation of Histone H2B at Serine 14 has been linked to transcriptional activation and is often associated with dynamic changes in chromatin structure.
By targeting this specific modification, researchers can gain insights into the mechanisms underlying gene regulation and cellular function.The Phospho-Histone H2B (Ser14) Antibody is an indispensable tool for researchers interested in epigenetics, chromatin remodeling, and gene expression regulation. Its high specificity and sensitivity enable accurate detection and quantification of Histone H2B phosphorylation, making it ideal for studies in cancer research, developmental biology, and stem cell biology. Gain a deeper understanding of histone modifications and their impact on cellular processes with this reliable and versatile antibody.
Antibody Name:
Phospho-Histone H2B (Ser14) Antibody
Antibody SKU:
PACO00172
Size:
50ug
Host Species:
Rabbit
Tested Applications:
WB
Recommended Dilutions:
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthetic Peptide
Form:
Liquid
Storage Buffer:
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
H2B1A: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Transcribed exclusively in testis, and the corresponding protein is also present in mature sperm. Belongs to the histone H2B family.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: extrinsic to plasma membrane; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleusMolecular Function: DNA binding; protein heterodimerization activityBiological Process: establishment and/or maintenance of chromatin architecture; inflammatory response; nucleosome assembly; nucleosome disassembly; plasminogen activation; positive regulation of binding; spermatogenesis, exchange of chromosomal proteins
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015]
