The Phospho-Histone H2A (S129) Rabbit Polyclonal Antibody (CABP1063) is an essential tool for researchers studying histone modifications and their role in chromatin dynamics. This antibody specifically targets the phosphorylated form of Histone H2A at serine 129, a crucial post-translational modification involved in DNA damage response and repair.Raised in rabbits, this antibody has been validated for use in Western blot applications and is highly reactive with human samples. Its ability to detect and analyze phosphorylated Histone H2A in various cell types makes it ideal for studies in epigenetics, DNA damage signaling, and cancer research.
Histone H2A phosphorylation at serine 129 is a key event in the cellular response to DNA damage, playing a role in chromatin relaxation, recruitment of repair factors, and checkpoint activation. Understanding the dynamics of this histone modification is essential for unraveling its contribution to genomic stability and disease development.Overall, the Phospho-Histone H2A (S129) Rabbit Polyclonal Antibody (CABP1063) offers a valuable tool for investigating the intricate mechanisms of chromatin remodeling and DNA damage response, paving the way for advancements in cancer therapy and personalized medicine.
A synthetic phosphorylated peptide around S129 of saccharomyces cerevisiae Histone H2A (NP_010511.3?).
Sequence:
KASQ E
Tested Applications:
IHC-PELISA
Recommended Dilution:
IHC-P,1:50 - 1:200
Synonyms:
H2A1; SPT11; Phospho-Histone H2A-S129
Cellular Localization:
nucleus
Calculated MW:
14kDa
Predicted to enable DNA binding activity. Involved in DNA repair; negative regulation of transcription by RNA polymerase II; and transfer RNA gene-mediated silencing. Part of replication fork protection complex. Orthologous to several human genes including H2AX (H2A.X variant histone).
Purification Method:
Affinity purification
Gene ID:
851811
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Immunohistochemistry analysis of paraffin-embedded Rat liver using Phospho-Histone H2A-S129 Rabbit pAb (CABP1063) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
