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Phospho-HIST1H1B (T154) Antibody (PACO56601)

SKU:
PACO56601
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
WB
IF
ChIP
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
  • Phospho-HIST1H1B T154 Antibody PACO56601
  • Phospho-HIST1H1B T154 Antibody PACO56601
  • Phospho-HIST1H1B T154 Antibody PACO56601
€349
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Description

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Phospho-HIST1H1B (T154) Antibody (PACO56601)

The Phospho-Hist1H1B (T154) Antibody (PAC05601) is a highly specific research tool designed for the study of Histone H1B phosphorylation at threonine 154. This antibody, produced in rabbits, is optimized for detection of phosphorylated Hist1H1B in human samples and has been validated for use in Western blot applications.Histone H1B is a key protein involved in chromatin structure and gene regulation, and phosphorylation at threonine 154 is known to play a role in various cellular processes, including cell division and DNA replication. The Phospho-Hist1H1B (T154) Antibody binds specifically to the phosphorylated form of Hist1H1B, enabling researchers to study the impact of this post-translational modification on histone function.

This antibody is an essential tool for researchers studying epigenetic regulation, chromatin dynamics, and cell cycle progression. By targeting the phosphorylated form of Hist1H1B, this antibody allows for precise detection and analysis of Hist1H1B phosphorylation, providing valuable insights into the mechanisms underlying cellular processes such as gene expression and chromatin remodeling.

Antibody Name:Phospho-HIST1H1B (T154) Antibody (PACO56601)
Antibody SKU:PACO56601
Size:50ul
Host Species:Rabbit
Tested Applications:ELISA, WB, IF, ChIP
Recommended Dilutions:ELISA:1:2000-1:10000, WB:1:200-1:2000, IF:1:50-1:200
Species Reactivity:Human
Immunogen:Peptide sequence around site of Phospho-Thr (154) derived from Human Histone H1.5
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method:Antigen Affinity Purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Product ImageWestern Blot. Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate. All lanes: HIST1H1B antibody at 1.5µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 23 kDa. Observed band size: 32 kDa.
Product ImageImmunofluorescence staining of HepG2 cells with PACO56601 at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Product ImageChromatin Immunoprecipitation Hela (4*106, treated with 100nM calyculin A for 60min) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H1B (PACO56601) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the beta -Globin promoter.
Background:Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
Synonyms:Histone H1.5 (Histone H1a) (Histone H1b) (Histone H1s-3), HIST1H1B, H1F5
UniProt Protein Function:H1B: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 6p22.1

Cellular Component: nuclear chromatin; nuclear heterochromatin; nucleosome

Molecular Function:chromatin DNA binding; histone deacetylase binding

Biological Process: establishment and/or maintenance of chromatin architecture; negative regulation of transcription from RNA polymerase II promoter; nucleosome assembly; positive regulation of cell growth; positive regulation of histone H3-K9 methylation; protein stabilization

NCBI Summary:Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
UniProt Code:P16401
NCBI GenInfo Identifier:19856407
NCBI Gene ID:3009
NCBI Accession:P16401.3
UniProt Secondary Accession:P16401,Q14529, Q3MJ42,
UniProt Related Accession:P16401
Molecular Weight:22,580 Da
NCBI Full Name:Histone H1.5
NCBI Synonym Full Names:histone cluster 1, H1b
NCBI Official Symbol:HIST1H1B  
NCBI Official Synonym Symbols:H1; H1B; H1.5; H1F5; H1s-3  
NCBI Protein Information:histone H1.5
UniProt Protein Name:Histone H1.5
UniProt Synonym Protein Names:Histone H1a; Histone H1b; Histone H1s-3
Protein Family:Histone
UniProt Gene Name:HIST1H1B  
UniProt Entry Name:H15_HUMAN
Secondary Antibody
Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)
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