The Phospho-HDAC5 (S498) Polyclonal Antibody (CABP0202) offered by Assay Genie is specifically designed for researchers studying the phosphorylation of HDAC5 at serine 498. This antibody, produced in rabbits, is highly reactive with human samples and has been validated for use in Western blot applications.HDAC5 is a member of the histone deacetylase family and plays a crucial role in the regulation of gene expression through the modification of histones. Phosphorylation of HDAC5 at serine 498 has been implicated in various cellular processes, including cell cycle regulation, apoptosis, and cell differentiation.
By using the Phospho-HDAC5 (S498) Polyclonal Antibody, researchers can accurately detect and analyze the phosphorylation status of HDAC5 in a variety of cell types. This antibody is an invaluable tool for studies in the fields of epigenetics, cell biology, and cancer research, providing insights into the role of HDAC5 phosphorylation in cellular function and disease development.
Product Name:
Phospho-HDAC5-S498 Rabbit Polyclonal Antibody
SKU:
CABP0202
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S498 of human HDAC5 (NP_005465.2).
Sequence:
TQSS P
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
HD5; NY-CO-9; Phospho-HDAC5-S498
Positive Sample:
NIH/3T3,NIH/3T3+Insulin
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
122kDa
Observed MW:
140kDa
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the class II histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. This gene is thought to be associated with colon cancer. Two transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
10014
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3 cells, using Phospho-HDAC5-S498 antibody (CABP0202) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 30s.
