The Phospho-HDAC4 (S632) Monoclonal Antibody is a valuable tool for researchers studying histone deacetylase 4 (HDAC4) phosphorylation at serine 632, a critical post-translational modification that regulates its activity. This antibody, generated through monoclonal antibody technology, is highly specific to the phosphorylated form of HDAC4 and has been validated for use in various experimental techniques, including Western blotting and immunohistochemistry.HDAC4 is a key player in epigenetic regulation, influencing gene expression by removing acetyl groups from histone proteins. Phosphorylation of HDAC4 at serine 632 is known to impact its subcellular localization and function, making it a crucial site for studying the regulation of gene transcription. By targeting the phosphorylated form of HDAC4, researchers can explore its involvement in various cellular processes, including cell differentiation, proliferation, and apoptosis.
This Phospho-HDAC4 (S632) Monoclonal Antibody offers researchers a reliable tool for investigating the role of HDAC4 phosphorylation in cellular signaling pathways and disease progression. Its high specificity and sensitivity make it an ideal choice for studies focused on understanding the molecular mechanisms underlying cancer, neurodegenerative disorders, and cardiovascular diseases. By utilizing this antibody, researchers can gain valuable insights into the complex regulatory networks controlled by HDAC4 phosphorylation, advancing our knowledge of epigenetic mechanisms in health and
Product Name:
Phospho-HDAC4-S632 Monoclonal Antibody
SKU:
CABP1344
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S632 of human Phospho-HDAC4-S632 (NP_006028.2).
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.
Purification Method:
Affinity purification
Gene ID:
9759
Clone Number:
ARC56258
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of Jurkat cells, using Phospho-HDAC4-S632 antibody (CABP1344) at1:2000 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
