The Phospho-HDAC4 (S246)/HDAC5 (S259)/HDAC9 (S220) Polyclonal Antibody (CABP0280) is a valuable tool for researchers studying the phosphorylation of HDAC proteins, which play crucial roles in epigenetic regulation and gene expression. This antibody, raised in rabbits, is highly specific for detecting phosphorylated HDAC4 at Ser246, HDAC5 at Ser259, and HDAC9 at Ser220 in human samples.Phosphorylation of HDAC proteins can impact their function, leading to changes in chromatin structure and gene expression patterns. The ability to detect and quantify phosphorylated forms of HDAC4, HDAC5, and HDAC9 is essential for understanding their regulatory roles in various cellular processes, including cell cycle regulation, differentiation, and apoptosis.
This polyclonal antibody is validated for use in Western blot applications and is suitable for detecting phosphorylated HDAC4, HDAC5, and HDAC9 in a variety of cell types and experimental conditions. It is an essential tool for researchers investigating the roles of HDAC proteins in epigenetic regulation, cell signaling pathways, and disease pathogenesis. Order now to advance your research in the fields of molecular biology, cancer biology, and epigenetics.
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
9759/10014/9734
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 cells untreated or treated with EGF using Phospho-HDAC4-S246/HDAC5-S259/HDAC9-S220 Rabbit pAb (CABP0280).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
