The Phospho-HDAC2 (S394) Polyclonal Antibody (CABP0201) is a valuable tool for research involving HDAC2, a key player in epigenetic regulation. This antibody, generated in rabbits, is highly specific to human samples and has been validated for use in Western blot applications. It targets the phosphorylated form of HDAC2 at Serine 394, allowing for precise detection and analysis in various cell types.HDAC2, a member of the histone deacetylase family, is involved in chromatin remodeling and gene expression regulation. Phosphorylation of HDAC2 at S394 has been linked to its activity in various cellular processes, including cell cycle regulation and DNA damage response.
Understanding the regulation and function of phosphorylated HDAC2 is essential for unraveling its role in cancer, neurodegenerative diseases, and other pathological conditions.Researchers studying epigenetics, cell signaling, and chromatin dynamics will find the Phospho-HDAC2 (S394) Polyclonal Antibody to be a valuable asset in their investigations. Its high specificity and sensitivity make it ideal for elucidating the intricate mechanisms underlying HDAC2 phosphorylation and its impact on cellular processes.
Product Name:
Phospho-HDAC2-S394 Polyclonal Antibody
SKU:
CABP0201
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A phospho specific peptide corresponding to residues surrounding S394 of human HDAC2
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:100 - 1:500 IHC-P,1:50 - 1:200
Synonyms:
HD2; RPD3; YAF1; KDAC2; Phospho-HDAC2-S394
Positive Sample:
HepG2
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
55kDa
Observed MW:
62kDa
This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
3066
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Phospho-HDAC2-S394 Rabbit pAb (CABP0201) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
