The Phospho-H2AX (S139) Rabbit Monoclonal Antibody (CABP0687) is a valuable tool for researchers studying DNA damage and repair processes. This antibody specifically targets the phosphorylated form of histone H2AX at serine 139, a marker of double-strand DNA breaks.Phosphorylation of H2AX at serine 139 is a key event in the cellular response to DNA damage, triggering a cascade of events that recruit repair factors to the site of damage. This antibody, raised in rabbits, is highly sensitive and specific for detecting phosphorylated H2AX in various cell types and is validated for use in Western blot and immunofluorescence applications.
By using the Phospho-H2AX (S139) Rabbit Monoclonal Antibody, researchers can investigate the dynamics of DNA damage and repair processes, elucidate the mechanisms involved, and identify potential targets for therapeutic intervention in conditions such as cancer, genetic disorders, and neurodegenerative diseases. This antibody is a valuable tool for advancing our understanding of DNA damage response pathways and developing novel strategies for diagnosing and treating DNA repair-related disorders.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
Purification Method:
Affinity purification
Gene ID:
3014
Clone Number:
ARC0110
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Histone H2AX-S139 Rabbit mAb (CABP0687) at 1:1000 dilution.NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
