The Phospho-GSK3B (Y216) Polyclonal Antibody (CABP0512) is a valuable tool for researchers studying GSK3B, a key regulator of cell signaling pathways involved in cell proliferation, differentiation, and apoptosis. This antibody, produced in rabbits, is specifically designed to target the phosphorylated form of GSK3B at tyrosine 216, allowing for precise detection and quantification in Western blot assays.GSK3B is known to play a crucial role in various cellular processes, including glycogen metabolism, gene transcription, and cell cycle regulation. Phosphorylation of GSK3B at tyrosine 216 has been linked to its activation and modulation of downstream signaling cascades, making this site an important target for investigation in cancer biology, neurodegenerative diseases, and metabolic disorders.
By using the Phospho-GSK3B (Y216) Polyclonal Antibody, researchers can gain insights into the mechanisms underlying GSK3B-dependent cellular functions and explore potential therapeutic strategies targeting this signaling molecule. Its high specificity for phosphorylated GSK3B at tyrosine 216 ensures reliable and reproducible results, making it a valuable tool for studies aimed at understanding the role of GSK3B in health and disease.
A synthetic phosphorylated peptide around Y216 of human GSK3β (NP_001139628).
Sequence:
VSYI C
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
GSK3B; gsk-3β; Phospho-GSK3β-Y216 + GSK3α-Y279
Positive Sample:
293
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Nucleus.
Calculated MW:
47kDa
Observed MW:
50kDa
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2931/2932
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-GSK3β-Y216 + GSK3α-Y279 antibody (CABP0512) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by 10% FBS for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
