Phospho-GSK3β-S9 Rabbit Polyclonal Antibody (CABP0039)
- SKU:
- CABP0039
- Product Type:
- Antibody
- Applications:
- WB
- IHC
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Product Name: | Phospho-GSK3β-S9 Rabbit Polyclonal Antibody |
| SKU: | CABP0039 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human,Mouse,Rat |
| Immunogen: | A synthetic phosphorylated peptide around S9 of human GSK3β (NP_001139628.1). |
| Sequence: | TTSF AE |
| Tested Applications: | WB IHC-P IF/ICC ELISA |
| Recommended Dilution: | WB,1:100 - 1:500 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 |
| Synonyms: | GSK3B; gsk-3β; Phospho-GSK3β-S9 |
| Positive Sample: | C6 |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 47kDa |
| Observed MW: | 46 kDa |
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
| Purification Method: | Affinity purification |
| Gene ID: | 2932 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
 | Western blot analysis of lysates from C6 cells using Phospho-GSK3β-S9 Rabbit pAb (CABP0039) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s. |
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