The Phospho-GSK3B (S9) Polyclonal Antibody (CABP0039) is a valuable tool for researchers studying the phosphorylation of GSK3B at serine 9, a key regulatory site involved in various cellular processes. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of GSK3B, this antibody allows for the detection and analysis of GSK3B activation in different cell types, making it an essential reagent for investigations in cell signaling, metabolism, and cancer biology.
GSK3B, a serine/threonine kinase, is a crucial player in multiple signaling pathways that regulate cell growth, proliferation, and survival. Phosphorylation of GSK3B at serine 9 is known to inhibit its activity, leading to downstream effects on various cellular functions. The Phospho-GSK3B (S9) Polyclonal Antibody provides researchers with a specific tool to study the activation status of GSK3B and its impact on cellular processes, making it a valuable asset in the fields of molecular biology, biochemistry, and drug discovery.
Product Name:
Phospho-GSK3β-S9 Rabbit Polyclonal Antibody
SKU:
CABP0039
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S9 of human GSK3β (NP_001139628.1).
The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease.
Purification Method:
Affinity purification
Gene ID:
2932
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from C6 cells using Phospho-GSK3β-S9 Rabbit pAb (CABP0039) at 1:500 dilution. C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
