The Phospho-GSK3A (S21) Polyclonal Antibody (CABP0582) is a valuable tool for researchers studying the regulation of Glycogen Synthase Kinase-3 Alpha (GSK3A) phosphorylation at serine 21. This antibody, raised in rabbits, is highly specific to human samples and has been validated for use in Western blot applications.GSK3A is a key regulator of various cellular processes, including cell proliferation, differentiation, and apoptosis. Phosphorylation of GSK3A at serine 21 is known to inhibit its activity, impacting downstream signaling pathways involved in cell growth and survival. The Phospho-GSK3A (S21) Polyclonal Antibody enables detection and analysis of phosphorylated GSK3A in a variety of cell types, making it an ideal tool for studies in cell biology, cancer research, and drug development.
Understanding the phosphorylation status of GSK3A at serine 21 is crucial for unraveling its role in disease pathogenesis and identifying potential therapeutic targets for conditions such as cancer, neurodegenerative disorders, and inflammatory diseases. By utilizing the Phospho-GSK3A (S21) Polyclonal Antibody, researchers can gain valuable insights into the regulation of GSK3A phosphorylation and its impact on cellular function, paving the way for novel therapeutic strategies.
Product Name:
Phospho-GSK3α-S21 Rabbit Polyclonal Antibody
SKU:
CABP0582
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S21 of human GSK3α (NP_063937.2).
Sequence:
TSSF A
Tested Applications:
WBIPELISA
Recommended Dilution:
WB,1:500 - 1:2000 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
This gene encodes a multifunctional Ser/Thr protein kinase that is implicated in the control of several regulatory proteins including glycogen synthase, and transcription factors, such as JUN. It also plays a role in the WNT and PI3K signaling pathways, as well as regulates the production of beta-amyloid peptides associated with Alzheimer's disease.
Purification Method:
Affinity purification
Gene ID:
2931
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from 293 and HeLa cells, using Phospho-GSK3α-S21 Rabbit pAb (CABP0582) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
