Phospho-GSK3α-S21+GSK3β-S9 Monoclonal Antibody (CABP1341)
- SKU:
- CABP1341
- Product Type:
- Antibody
- Antibody Type:
- Monoclonal Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | Phospho-GSK3α-S21+GSK3β-S9 Monoclonal Antibody |
| SKU: | CABP1341 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Mouse |
| Immunogen: | A synthetic phosphorylated peptide around S21&S9 of human GSK3α/GSK3β (NP_063937.2/NP_002084.2). |
| Sequence: | TSSF A |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | GSK3B; GSK 3 beta; GSK3A; GSK 3 alpha; Phospho-GSK3α-S21+GSK3β-S9 |
| Positive Sample: | NIH/3T3+FBS |
| Conjugate: | Unconjugated |
| Cellular Localization: | Cell membrane, Cytoplasm, Nucleus. |
| Calculated MW: | 51kDa/46kDa |
| Observed MW: | 51kDa/46kDa |
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
| Purification Method: | Affinity purification |
| Gene ID: | 2931/2932 |
| Clone Number: | ARC54771 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3. |
 | Western blot analysis of extracts of NIH/3T3, using Phospho-GSK3α-S21+GSK3β-S9 antibody (CABP1341) at 1:800 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s. |
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