The Phospho-GSK3 (S21) + GSK3 (S9) Monoclonal Antibody is specifically designed for research involving GSK3, a protein kinase implicated in various cellular processes including cell proliferation, differentiation, and apoptosis. This antibody, produced in mice, is highly specific for detecting phosphorylation at serine 21 of GSK3 and serine 9 of GSK3, making it an ideal tool for studying the regulation of GSK3 activity in various biological contexts.GSK3 is a key player in multiple signaling pathways, including those involved in cell survival, growth, and metabolism. Dysregulation of GSK3 activity has been linked to various diseases, including cancer, neurodegenerative disorders, and diabetes.
Understanding the phosphorylation status of GSK3 at serine 21 and serine 9 can provide valuable insights into the role of GSK3 in these diseases and may lead to the development of targeted therapies.The Phospho-GSK3 (S21) + GSK3 (S9) Monoclonal Antibody is validated for use in Western blot applications and is highly reactive with human samples. Its specificity for phosphorylated GSK3 at serine 21 and serine 9 makes it a powerful tool for investigating the regulation of GSK3 activity and its implications in disease pathology. Whether studying basic cell signaling mechanisms or exploring therapeutic targets, this antibody is essential for researchers in the fields of cell
Product Name:
Phospho-GSK3α-S21+GSK3β-S9 Monoclonal Antibody
SKU:
CABP1341
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around S21&S9 of human GSK3α/GSK3β (NP_063937.2/NP_002084.2).
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2931/2932
Clone Number:
ARC54771
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of NIH/3T3, using Phospho-GSK3α-S21+GSK3β-S9 antibody (CABP1341) at 1:800 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
