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Anti-Phospho-GRIN2B-Y1474 pAb Antibody (CABP0771)

SKU:
CABP0771
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Signal Transduction
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Description

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Antibody Name:Anti-Phospho-GRIN2B-Y1474 Antibody
Antibody SKU:CABP0771
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic phosphorylated peptide around Y1474 of human GRIN2B (NP_000825.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:NIH/3T3
Immunogen:A synthetic phosphorylated peptide around Y1474 of human GRIN2B (NP_000825.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HVYE K
Gene ID:2904
Uniprot:Q13224
Cellular Location:Cell junction, Cell membrane, Multi-pass membrane protein, postsynaptic cell membrane, synapse
Calculated MW:166kDa
Observed MW:190KDa
Anti-Phospho-GRIN2B-Y1474 pAb (CABP0771)Western blot analysis of extracts of various cell lines, using Anti-Phospho-GRIN2B-Y1474 pAb (CABP0771) at 1:1000 dilution.NIH/3T3 cells were treated by EGF (100 ng/mL) at 37'C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Synonyms:GRIN2B, EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, hNR3
Background:N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
UniProt Protein Function:NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.
UniProt Protein Details:

Protein type:Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p12

Cellular Component: postsynaptic membrane; synaptic vesicle; neuron projection; cell surface; integral to plasma membrane; dendrite; plasma membrane; cell junction; N-methyl-D-aspartate selective glutamate receptor complex

Molecular Function:protein binding; extracellular-glutamate-gated ion channel activity; zinc ion binding; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; calcium channel activity

Biological Process: synaptic transmission, glutamatergic; axon guidance; startle response; behavioral fear response; in utero embryonic development; glutamate signaling pathway; regulation of synaptic plasticity; learning; memory; synaptic transmission; detection of mechanical stimulus involved in sensory perception of pain; behavioral response to pain; sensory organ development; learning and/or memory; response to ethanol; suckling behavior; transport; ionotropic glutamate receptor signaling pathway; ephrin receptor signaling pathway; regulation of excitatory postsynaptic membrane potential

Disease: Mental Retardation, Autosomal Dominant 6; Epileptic Encephalopathy, Early Infantile, 27

NCBI Summary:N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]
UniProt Code:Q13224
NCBI GenInfo Identifier:14548162
NCBI Gene ID:2904
NCBI Accession:Q13224.3
UniProt Secondary Accession:Q13224,Q12919, Q13220, Q13225, Q14CU4, Q9UM56,
UniProt Related Accession:Q13224
Molecular Weight:1484
NCBI Full Name:Glutamate receptor ionotropic, NMDA 2B
NCBI Synonym Full Names:glutamate receptor, ionotropic, N-methyl D-aspartate 2B
NCBI Official Symbol:GRIN2B  
NCBI Official Synonym Symbols:MRD6; NR2B; hNR3; EIEE27; GluN2B; NMDAR2B  
NCBI Protein Information:glutamate receptor ionotropic, NMDA 2B; NR3; glutamate receptor subunit epsilon-2; N-methyl-D-aspartate receptor subunit 3; N-methyl D-aspartate receptor subtype 2B; glutamate [NMDA] receptor subunit epsilon-2
UniProt Protein Name:Glutamate receptor ionotropic, NMDA 2B
UniProt Synonym Protein Names:Glutamate [NMDA] receptor subunit epsilon-2; N-methyl D-aspartate receptor subtype 2B; NMDAR2B; NR2B; N-methyl-D-aspartate receptor subunit 3; NR3; hNR3
Protein Family:Glutamate receptor ionotropic
UniProt Gene Name:GRIN2B  
UniProt Entry Name:NMDE2_HUMAN

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