The Phospho-GCN2 (T899) Polyclonal Antibody is a valuable tool for researchers studying the GCN2 protein, a crucial regulator of cellular stress responses. This antibody, raised in rabbits, demonstrates high reactivity with human samples and is specifically validated for use in Western blot applications. By targeting the phosphorylated form of GCN2 at threonine 899, this antibody allows for the precise detection and quantification of this important protein in various cell types.GCN2, also known as General Control Non-derepressible 2, is a key player in the integrated stress response pathway that helps cells adapt to environmental stressors such as nutrient deprivation. Phosphorylation of GCN2 at threonine 899 is a crucial event in its activation and signaling cascade.
Research on GCN2 is essential for understanding how cells respond to stress and maintain homeostasis, making this antibody a valuable tool for studies in molecular biology, cell biology, and cancer research.Investigating the role of GCN2 and its phosphorylation at threonine 899 can provide insights into the mechanisms underlying cellular stress responses and potential therapeutic targets for diseases associated with dysregulated stress signaling pathways. The Phospho-GCN2 (T899) Polyclonal Antibody offers researchers the precision and sensitivity needed to advance their understanding of GCN2 biology and its implications for human health.
Product Name:
Phospho-GCN2-T899 Polyclonal Antibody
SKU:
CABP1428
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T899 of human Phospho-GCN2-T899.
Sequence:
HLTG M
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:1000
Synonyms:
GCN2; PVOD2; Phospho-GCN2-T899
Positive Sample:
HeLa
Conjugate:
Unconjugated
Cellular Localization:
cytosol, cytosolic ribosome
Calculated MW:
187kDa
Observed MW:
187kDa
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
Purification Method:
Affinity purification
Gene ID:
440275
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of HeLa, using Phospho-GCN2-T899 Rabbit pAb (CABP1428) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
