Phospho-GCN2-T899 Polyclonal Antibody (CABP1428)
- SKU:
- CABP1428
- Product Type:
- Antibody
- Antibody Type:
- Polyclonal Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- WB
Frequently bought together:
Description
| Product Name: | Phospho-GCN2-T899 Polyclonal Antibody |
| SKU: | CABP1428 |
| Size: | 20uL, 100uL |
| Isotype: | IgG |
| Host Species: | Rabbit |
| Reactivity: | Human |
| Immunogen: | A synthetic phosphorylated peptide around T899 of human Phospho-GCN2-T899. |
| Sequence: | HLTG M |
| Tested Applications: | WB ELISA |
| Recommended Dilution: | WB,1:500 - 1:1000 |
| Synonyms: | GCN2; PVOD2; Phospho-GCN2-T899 |
| Positive Sample: | HeLa |
| Conjugate: | Unconjugated |
| Cellular Localization: | cytosol, cytosolic ribosome |
| Calculated MW: | 187kDa |
| Observed MW: | 187kDa |
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
| Purification Method: | Affinity purification |
| Gene ID: | 440275 |
| Storage Buffer: | Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3. |
 | Western blot analysis of HeLa, using Phospho-GCN2-T899 Rabbit pAb (CABP1428) at 1:1000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s. |
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