The Phospho-GCN2 (T899) Monoclonal Antibody is a specialized tool for researchers studying the GCN2 protein, a key regulator of cellular stress response and amino acid metabolism. This antibody, developed using advanced monoclonal antibody technology, specifically recognizes the phosphorylated form of GCN2 at threonine 899 residue in human samples.GCN2 is a conserved kinase that plays a crucial role in integrating amino acid availability with protein synthesis through the regulation of the eIF2α signaling pathway. Phosphorylation of GCN2 at threonine 899 is known to modulate its activity and influence downstream signaling cascades involved in stress responses, autophagy, and immune function.
The Phospho-GCN2 (T899) Monoclonal Antibody offers high specificity and sensitivity for detecting and quantifying phosphorylated GCN2 in various experimental settings, such as Western blot analysis. This enables researchers to investigate the role of GCN2 in cellular stress, nutrient sensing, and metabolic regulation, providing insights into the molecular mechanisms underlying diseases like cancer, metabolic disorders, and neurodegenerative conditions. Unlock the potential of GCN2 research with this cutting-edge antibody tool.
Product Name:
Phospho-GCN2-T899 Monoclonal Antibody
SKU:
CABP1356
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around T899 of human Phospho-GCN2-T899 (NP_001013725.2).
Sequence:
HLTG M
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:1000 - 1:5000
Synonyms:
GCN2; PVOD2; Phospho-GCN2-T899
Positive Sample:
HeLa+Calyculin A
Conjugate:
Unconjugated
Cellular Localization:
Cytosol, Cytosolic ribosome
Calculated MW:
187kDa
Observed MW:
220kDa
This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2.
Purification Method:
Affinity purification
Gene ID:
440275
Clone Number:
ARC57177
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-GCN2-T899 antibody (CABP1356) at1:2000 dilution.HeLa cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
