Anti-Phospho-GAB1-Y659 pAb Antibody (CABP0774)
- SKU:
- CABP0774
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
Anti-Phospho-GAB1-Y659 pAb Antibody (CABP0774)
The Phospho-GAB1 (Y659) Polyclonal Antibody (CABP0774) is a valuable tool for researchers studying the GAB1 protein, which plays a key role in cell signaling pathways, particularly in the regulation of cell growth, survival, and differentiation. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the phosphorylated form of GAB1 at tyrosine 659, this antibody enables the detection and analysis of GAB1 activation in various cell types.The phosphorylation of GAB1 at tyrosine 659 is known to be involved in various cellular processes, including cell proliferation, migration, and survival. Dysregulation of GAB1 phosphorylation has been linked to cancer development and progression, making it a promising target for cancer research and therapy development.
By studying the phosphorylation status of GAB1, researchers can gain insights into its role in cancer biology and potentially uncover new therapeutic strategies for treating cancer.In conclusion, the Phospho-GAB1 (Y659) Polyclonal Antibody (CABP0774) is a valuable tool for investigating the signaling pathways involving GAB1 and its role in cancer biology. Its high reactivity and specificity make it an ideal choice for researchers looking to study the phosphorylation status of GAB1 and its implications in cancer development and progression.
| Antibody Name: | Anti-Phospho-GAB1-Y659 Antibody |
| Antibody SKU: | CABP0774 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic phosphorylated peptide around Y659 of human GAB1 (NP_002030.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | NIH/3T3 |
| Immunogen: | A synthetic phosphorylated peptide around Y659 of human GAB1 (NP_002030.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VDYV V |
| Gene ID: | 2549 |
| Uniprot: | Q13480 |
| Cellular Location: | |
| Calculated MW: | 76kDa/80kDa |
| Observed MW: | 110kDa |
 | Western blot analysis of extracts of NIH/3T3 cells, using Phospho-GAB1-Y659 antibody (CABP0774) at 1:2000 dilution. NIH/3T3 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
| Synonyms: | GAB1, GRB2 |
| Background: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | GAB1: Adapter protein that plays a role in intracellular signaling cascades triggered by activated receptor-type kinases. Plays a role in FGFR1 signaling. Probably involved in signaling by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2. Interacts with PTPRJ. Identified in a complex containing FRS2, GRB2, GAB1, PIK3R1 and SOS1. Interacts (phosphorylated) with PTPN11. Interacts with HCK. Belongs to the GAB family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 4q31.21 Cellular Component: cytosol Molecular Function:protein binding; SH3/SH2 adaptor activity; signal transducer activity Biological Process: activation of JNK activity; cell proliferation; epidermal growth factor receptor signaling pathway; epidermis development; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; insulin receptor signaling pathway; nerve growth factor receptor signaling pathway; phosphoinositide-mediated signaling; platelet-derived growth factor receptor signaling pathway; regulation of cell migration; response to oxidative stress |
| NCBI Summary: | The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. It is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008] |
| UniProt Code: | Q13480 |
| NCBI GenInfo Identifier: | 90180201 |
| NCBI Gene ID: | 2549 |
| NCBI Accession: | Q13480.2 |
| UniProt Secondary Accession: | Q13480,Q4W5G2, Q6P1W2, A8K152, |
| UniProt Related Accession: | Q13480 |
| Molecular Weight: | 80,005 Da |
| NCBI Full Name: | GRB2-associated-binding protein 1 |
| NCBI Synonym Full Names: | GRB2 associated binding protein 1 |
| NCBI Official Symbol: | GAB1Â Â |
| NCBI Protein Information: | GRB2-associated-binding protein 1 |
| UniProt Protein Name: | GRB2-associated-binding protein 1 |
| UniProt Synonym Protein Names: | GRB2-associated binder 1; Growth factor receptor bound protein 2-associated protein 1 |
| Protein Family: | GPI transamidase component |
| UniProt Gene Name: | GAB1Â Â |
| UniProt Entry Name: | GAB1_HUMAN |
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