The Phospho-Fyn (S21) Polyclonal Antibody (CABP0510) is a valuable tool for researchers studying the phosphorylation of Fyn, a protein tyrosine kinase involved in various cellular processes. This antibody, produced in rabbits, shows high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the phosphorylated form of Fyn at Serine 21, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for investigations in signal transduction pathways and cancer research.Fyn is a member of the Src family of kinases and is known to play a role in cell growth, differentiation, and immune responses.
Phosphorylation of Fyn at Serine 21 has been implicated in the regulation of its activity, linking it to signaling events that control cell proliferation and survival. Understanding the phosphorylation status of Fyn is essential for unraveling its role in disease pathogenesis, particularly in cancer and neurodegenerative disorders. This antibody provides a reliable tool for studying the intricate signaling mechanisms involving Fyn phosphorylation and its potential as a therapeutic target.
Product Name:
Phospho-Fyn-S21 Rabbit Polyclonal Antibody
SKU:
CABP0510
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S21 of human Phospho-Fyn-S21 (NP_002028.1).
Sequence:
DGSL N
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
SLK; SYN; p59-FYN; Phospho-Fyn-S21
Positive Sample:
A-431
Conjugate:
Unconjugated
Cellular Localization:
Cell membrane, Cytoplasm, Nucleus.
Calculated MW:
61kDa
Observed MW:
60kDa
This gene is a member of the protein-tyrosine kinase oncogene family. It encodes a membrane-associated tyrosine kinase that has been implicated in the control of cell growth. The protein associates with the p85 subunit of phosphatidylinositol 3-kinase and interacts with the fyn-binding protein. Alternatively spliced transcript variants encoding distinct isoforms exist.
Purification Method:
Affinity purification
Gene ID:
2534
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-Fyn-S21 Rabbit pAb (CABP0510) at 1:1000 dilution. HL-60 cells were treated by 10% FBS after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
