The Phospho-FOXO4 (S197) Polyclonal Antibody (CABP0177) is a valuable tool for researchers studying the phosphorylation of FOXO4, a transcription factor known for its role in regulating cellular processes such as apoptosis and cell cycle progression. This antibody, raised in rabbits, specifically detects phosphorylation of FOXO4 at serine 197 in human samples, making it an ideal choice for Western blot applications.FOXO4 is a key player in the regulation of cell survival and proliferation, and its phosphorylation at serine 197 is known to impact its activity and subcellular localization. By targeting this specific phosphorylation site, researchers can gain insights into the signaling pathways that control FOXO4-mediated cellular functions.
This antibody enables accurate detection and quantification of phosphorylated FOXO4 in various cell types, providing valuable data for studies in cancer biology, aging, and metabolic disorders.Overall, the Phospho-FOXO4 (S197) Polyclonal Antibody offers a reliable tool for investigating the role of FOXO4 phosphorylation in cellular processes and disease pathogenesis. Its high reactivity and specificity make it a valuable asset for researchers aiming to elucidate the complex regulatory mechanisms that govern FOXO4 activity in health and disease.
Product Name:
Phospho-FOXO4-S197 Rabbit Polyclonal Antibody
SKU:
CABP0177
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S197 of human FOXO4 (NP_005929.2).
Sequence:
AASM D
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:100 - 1:500 IF/ICC,1:100 - 1:200
Synonyms:
AFX; AFX1; MLLT7; Phospho-FOXO4-S197
Positive Sample:
293F,Jurkat
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
54kDa
Observed MW:
65kDa
This gene encodes a member of the O class of winged helix/forkhead transcription factor family. Proteins encoded by this class are regulated by factors involved in growth and differentiation indicating they play a role in these processes. A translocation involving this gene on chromosome X and the homolog of the Drosophila trithorax gene, encoding a DNA binding protein, located on chromosome 11 is associated with leukemia. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
4303
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Phospho-FOXO4-S197 antibody (CABP0177) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
