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Phospho-FOXO3A-S294 Rabbit Polyclonal Antibody (CABP0856)

SKU:
CABP0856
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death
$503
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-FOXO3A-S294 Rabbit Polyclonal Antibody
SKU:CABP0856
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic phosphorylated peptide around S294 of human FOXO3A (NP_001446.1).
Sequence:PGSP T
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2; Phospho-FOXO3A-S294
Positive Sample:NIH/3T3
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus, cytosol.
Calculated MW:71kDa
Observed MW:82kDa

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.

Purification Method:Affinity purification
Gene ID:2309
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO3A-S294 Rabbit pAb (CABP0856) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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