The Phospho-FoxO3 (S294) Polyclonal Antibody (CABP0856) is a valuable tool for researchers studying the phosphorylation of FoxO3 at serine 294, a key post-translational modification involved in the regulation of cellular processes such as apoptosis, cell cycle progression, and oxidative stress response. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.FoxO3 is a member of the Forkhead box O (FoxO) family of transcription factors that play important roles in cell proliferation, differentiation, and survival. Phosphorylation of FoxO3 at serine 294 has been linked to its subcellular localization and activity, making it a crucial site for understanding the regulation of FoxO3-dependent gene expression.
By targeting the phosphorylation of FoxO3 at serine 294, researchers can gain insights into the signaling pathways that control FoxO3 activity and its downstream effects on cellular function. This antibody is an essential tool for studies investigating the role of FoxO3 phosphorylation in diseases such as cancer, diabetes, and neurodegenerative disorders, where dysregulation of FoxO3 signaling pathways has been implicated.
Product Name:
Phospho-FOXO3A-S294 Rabbit Polyclonal Antibody
SKU:
CABP0856
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S294 of human FOXO3A (NP_001446.1).
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method:
Affinity purification
Gene ID:
2309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FOXO3A-S294 Rabbit pAb (CABP0856) at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
