The Phospho-FOXO3 (S253) Polyclonal Antibody (CABP0684) is a valuable tool for researchers studying FOXO3, a transcription factor involved in cell growth, apoptosis, and metabolism regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity when detecting phosphorylation of FOXO3 at serine 253. It has been validated for use in Western blot applications with human samples, allowing for precise quantification and analysis of FOXO3 phosphorylation in various cell types.FOXO3 is a key player in cellular processes such as cell cycle regulation, DNA damage repair, and oxidative stress response. Phosphorylation of FOXO3 at serine 253 is known to modulate its activity, influencing downstream gene expression and cellular responses.
Understanding the intricate regulatory mechanisms of FOXO3 phosphorylation can provide insights into diseases like cancer, neurodegenerative disorders, and metabolic syndromes.By utilizing the Phospho-FOXO3 (S253) Polyclonal Antibody, researchers can delve deeper into the functional roles of FOXO3 phosphorylation in disease pathogenesis and therapeutic development. This antibody is a valuable asset for studies in cell biology, molecular signaling, and drug discovery aimed at targeting FOXO3-related pathways for potential therapeutic interventions.
Product Name:
Phospho-FOXO3A-S253 Rabbit Polyclonal Antibody
SKU:
CABP0684
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S253 of human FOXO3A (NP_001446.1).
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
Purification Method:
Affinity purification
Gene ID:
2309
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells using Phospho-FOXO3A-S253 Rabbit pAb (CABP0684) at 1:500 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
