The Phospho-FOXO1 (T24), FOXO3A (T32), FOXO4 (T28) Polyclonal Antibody (CABP0892) is a valuable tool for researchers studying the FOXO family of transcription factors. These proteins play a crucial role in cellular processes such as metabolism, apoptosis, and cell cycle regulation. The antibody, generated in rabbits, has been validated for use in Western blot applications and is highly reactive with human samples.FOXO transcription factors are known targets of various signaling pathways, including the insulin-PI3K-Akt pathway, making them key players in the regulation of cell survival and proliferation. Phosphorylation of specific residues, such as T24 in FOXO1, T32 in FOXO3A, and T28 in FOXO4, can impact their activity and subcellular localization.
By using the Phospho-FOXO1 (T24), FOXO3A (T32), FOXO4 (T28) Polyclonal Antibody, researchers can investigate the phosphorylation status of these FOXO proteins under different conditions and gain insights into how they contribute to various cellular processes. This antibody is suitable for studies in cell biology, cancer research, and metabolic disorders, providing valuable information for understanding the intricate regulatory networks that control gene expression and cell function.
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
Purification Method:
Affinity purification
Gene ID:
2308/2309/4303
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Phospho-FOXO1-T24/FOXO3A-T32/FOXO4-T28 Rabbit pAb (CABP0892) at 1:500 dilution.Jurkat cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
