The Phospho-FOXO1 (S319) Rabbit Polyclonal Antibody (CABP1090) is a valuable tool for researchers studying FOXO1, a key transcription factor involved in regulating various cellular processes, including cell proliferation, apoptosis, and metabolism. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the phosphorylated form of FOXO1 at serine 319, this antibody enables detection and analysis of FOXO1 activation in a variety of cell types.FOXO1 is known to play a crucial role in various biological processes, including insulin signaling, oxidative stress response, and cell cycle regulation. Phosphorylation of FOXO1 at serine 319 specifically regulates its transcriptional activity, affecting downstream gene expression involved in cell growth and survival.
Understanding the phosphorylation status of FOXO1 can provide valuable insights into mechanisms regulating cellular functions, making this antibody a valuable tool for research in areas such as cancer biology, metabolic disorders, and aging-related studies.Overall, the Phospho-FOXO1 (S319) Rabbit Polyclonal Antibody (CABP1090) offers researchers a reliable and sensitive tool for investigating the role of FOXO1 phosphorylation in cellular processes and disease pathogenesis. Its specificity and reliability make it an essential component for studies aiming to elucidate the intricate regulatory mechanisms involving FOXO1 in various biological contexts.
Product Name:
Phospho-FOXO1-S319 Rabbit Polyclonal Antibody
SKU:
CABP1090
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around S319 of human FOXO1 (NP_002006.2).
Sequence:
TSSN A
Tested Applications:
WBIHC-PELISA
Recommended Dilution:
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200
Synonyms:
FKH1; FKHR; FOXO1A; Phospho-FOXO1-S319
Positive Sample:
HeLa
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
70kDa
Observed MW:
90kDa
This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma.
Purification Method:
Affinity purification
Gene ID:
2308
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using Phospho-FOXO1-S319 Rabbit pAb (CABP1090) at 1:1000 dilution or FOXO1 antibody (CAB13862).HeLa cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.