The Phospho-Fos (T232) Polyclonal Antibody (CABP0038) is a valuable tool for researchers studying the phospho-Fos protein, a transcription factor involved in cellular responses to various stimuli. This antibody, produced in rabbits, is highly specific for detecting phosphorylation at threonine 232 of the Fos protein and has been validated for use in Western blot applications. Its ability to target this specific phosphorylation site allows for precise analysis of signaling pathways and cellular processes in a variety of cell types.Phospho-Fos (T232) is a key player in the regulation of gene expression and cell proliferation, making it a crucial target for studies in cancer biology, cell signaling, and developmental biology.
By understanding the phosphorylation status of the Fos protein at threonine 232, researchers can gain insights into how this transcription factor influences cell behavior and potential implications for disease states. This antibody provides a powerful tool for investigating the role of phospho-Fos in various biological processes and disease pathways.
Product Name:
Phospho-c-Fos-T232 Rabbit Polyclonal Antibody
SKU:
CABP0038
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around T232 of human c-Fos (NP_005243.1).
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death.
Purification Method:
Affinity purification
Gene ID:
2353
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from HepG2 cells, using Phospho-c-Fos-T232 Rabbit pAb (CABP0038) at 1:1000 dilution or c-Fos antibody (CAB17351).HepG2 cells were treated by 10% FBS for after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.
