The Phospho-FLT3 (Y589/Y591) Polyclonal Antibody (CABP0529) is a valuable tool for researchers studying the FLT3 protein, a receptor tyrosine kinase involved in cell growth and differentiation. This antibody, produced in rabbits, is highly specific for phosphorylated FLT3 at tyrosine residues 589 and 591, which are important for FLT3 activation and signaling pathways.Validated for use in Western blot applications, this antibody enables the detection and analysis of phospho-FLT3 protein levels in various cell types. Its high reactivity with human samples makes it an ideal choice for studying FLT3 in immunology and cancer research.
FLT3 mutations are common in acute myeloid leukemia (AML) and are associated with poor prognosis. Understanding the phosphorylation status of FLT3 at specific tyrosine residues can provide insights into its aberrant activation in cancer and help in the development of targeted therapies. The Phospho-FLT3 (Y589/Y591) Polyclonal Antibody is a valuable tool for investigating the role of FLT3 in cancer biology and potential therapeutic interventions.
Product Name:
Phospho-FLT3-Y589/591 Rabbit Polyclonal Antibody
SKU:
CABP0529
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y589 & Y591 of human FLT3 (NP_004110.2).
Sequence:
EYFY V
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:100 - 1:500
Synonyms:
FLK2; STK1; CD135; FLK-2; Phospho-FLT3-Y589/591
Positive Sample:
A-431
Conjugate:
Unconjugated
Cellular Localization:
Endoplasmic reticulum lumen, Membrane, Single-pass type I membrane protein.
Calculated MW:
113kDa
Observed MW:
160kDa
This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Purification Method:
Affinity purification
Gene ID:
2322
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from A-431 cells, using Phospho-FLT3-Y589/591 Rabbit pAb (CABP0529) at 1:500 dilution.A-431 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
