The Phospho-FGFR3 (Y724) Polyclonal Antibody (CABP1274) is specifically designed for research involving FGFR3, a cell surface receptor involved in cell growth and development. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.FGFR3, a member of the fibroblast growth factor receptor family, plays a crucial role in signaling pathways that regulate cell proliferation, differentiation, and survival. Phosphorylation of FGFR3 at tyrosine 724 is known to be important for its activation and downstream signaling.
This antibody binds specifically to the phosphorylated form of FGFR3 at this site, allowing for the detection and analysis of FGFR3 activity in different cell types and tissues.Understanding the role of FGFR3 and its phosphorylation in various biological processes is essential for studying conditions like cancer, skeletal dysplasia, and developmental disorders. Research with this antibody can provide valuable insights into the mechanisms underlying these diseases and may lead to the development of targeted therapies that modulate FGFR3 signaling.
Product Name:
Phospho-FGFR3-Y724 Polyclonal Antibody
SKU:
CABP1274
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic phosphorylated peptide around Y724 of human FGFR3 (NP_000133.1).
Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Secreted, Single-pass type I membrane protein.
Calculated MW:
88kDa
Observed MW:
125kDa
This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia.
Purification Method:
Affinity purification
Gene ID:
2261
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from NIH/3T3 cells, using Phospho-FGFR3-Y724 Rabbit pAb (CABP1274) at 1:1000 dilution.NIH/3T3 cells were treated by pervanadate (1 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
