The Phospho-FGFR1 (Y653/Y654) Polyclonal Antibody is a valuable tool for researchers studying the activation of Fibroblast Growth Factor Receptor 1 (FGFR1) through phosphorylation at specific tyrosine residues. This antibody, generated in rabbits, exhibits high specificity and reactivity with human samples, making it an ideal choice for Western blot applications.FGFR1 is a transmembrane receptor tyrosine kinase that plays a crucial role in cell proliferation, differentiation, and survival. Phosphorylation of FGFR1 at tyrosine residues 653 and 654 is essential for its activation and downstream signaling, making it a key event in various cellular processes.
This antibody facilitates the detection and analysis of phosphorylated FGFR1, allowing researchers to investigate its role in cancer, development, and other physiological processes.By targeting the phosphorylated form of FGFR1, this antibody enables researchers to dissect the signaling pathways and functional implications associated with FGFR1 activation. Its use in immunology and cancer research provides valuable insights into the mechanisms regulating cell growth and survival, offering potential therapeutic targets for the treatment of cancer and other diseases.
Product Name:
Phospho-FGFR1-Y653/Y654 Polyclonal Antibody
SKU:
CABP1317
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic phosphorylated peptide around Y653 & Y654 of human Phospho-FGFR1-Y653/Y654 (NP_075598.2).
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Purification Method:
Affinity purification
Gene ID:
2260
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-FGFR1-Y653/Y654 antibody (CABP1317) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
