The Phospho-FGFR1 (Y653) Polyclonal Antibody (CABP0036) is a valuable tool for researchers studying the FGFR1 signaling pathway. This antibody, produced in rabbits, is highly specific for the phosphorylated tyrosine 653 residue of the FGFR1 protein and has been validated for use in Western blot and immunoprecipitation applications. By binding to the phosphorylated form of FGFR1, this antibody allows for the detection and analysis of FGFR1 activation in various cell types.FGFR1 is a receptor tyrosine kinase that plays a crucial role in cell proliferation, differentiation, and survival. Phosphorylation of specific tyrosine residues, such as Y653, is known to activate downstream signaling pathways that regulate cell growth and survival.
Dysregulation of FGFR1 signaling has been implicated in various diseases, including cancer, making it a promising target for therapeutic intervention.By utilizing the Phospho-FGFR1 (Y653) Polyclonal Antibody, researchers can gain insights into the activation status of FGFR1 in different cellular contexts, leading to a better understanding of its role in normal physiology and disease. This antibody is a valuable tool for studies in cancer biology, developmental biology, and signal transduction pathways involving FGFR1.
Product Name:
Phospho-FGFR1-Y653 Rabbit Polyclonal Antibody
SKU:
CABP0036
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic phosphorylated peptide around Y653 of human FGFR1 (NP_075598.2).
Cell membrane, Cytoplasm, Cytoplasmic vesicle, Nucleus, Single-pass type I membrane protein, cytosol.
Calculated MW:
92kDa
Observed MW:
120kDa/145kDa
The protein encoded by this gene is a member of the fibroblast growth factor receptor (FGFR) family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene have been associated with Pfeiffer syndrome, Jackson-Weiss syndrome, Antley-Bixler syndrome, osteoglophonic dysplasia, and autosomal dominant Kallmann syndrome 2. Chromosomal aberrations involving this gene are associated with stem cell myeloproliferative disorder and stem cell leukemia lymphoma syndrome. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Purification Method:
Affinity purification
Gene ID:
2260
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of 293T cells, using Phospho-FGFR1-Y653 pAb (CABP0036) at 1:1000 dilution.293T cells were treated by EGF (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
