The Phospho-FAK (Y397) Monoclonal Antibody is a valuable tool for researchers studying focal adhesion kinase (FAK) signaling pathways. This antibody, developed by AssayGenie, specifically targets the phosphorylated form of FAK at the tyrosine 397 residue, which is critical for FAK activation and downstream signaling cascades.The Phospho-FAK (Y397) Monoclonal Antibody is highly specific and sensitive, making it ideal for use in techniques such as Western blotting, immunoprecipitation, and immunofluorescence. Its ability to detect phosphorylated FAK allows for the precise analysis of FAK activation in cell and tissue samples, providing insight into cell migration, proliferation, and survival mechanisms.
Researchers in the fields of cancer biology, cell biology, and signal transduction will find the Phospho-FAK (Y397) Monoclonal Antibody to be a valuable asset in their studies. By investigating the role of FAK phosphorylation in various cellular processes, this antibody can help uncover new therapeutic targets for diseases such as cancer and fibrosis. Trust AssayGenie's Phospho-FAK (Y397) Monoclonal Antibody to enhance your research and advance our understanding of FAK signaling in health and disease.
Product Name:
Phospho-FAK-Y397 Monoclonal Antibody
SKU:
CABP1447
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around Y397 of human FAK（NP_722560.1）.
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
5747
Clone Number:
ARC62271
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of A-549, using Phospho-FAK-Y397 Rabbit mAb (CABP1447) at 1:4000 dilution.A-549 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
