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Phospho-FAK-Y397 Monoclonal Antibody (CABP1447)

SKU:
CABP1447
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
WB
€139 - €609
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-FAK-Y397 Monoclonal Antibody
SKU:CABP1447
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around Y397 of human FAK（NP_722560.1）.
Sequence:DDYA EI
Tested Applications:WB ELISA
Recommended Dilution:WB,1:2000 - 1:4000
Synonyms:FAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK; Phospho-FAK-Y397
Positive Sample:A-549
Conjugate:Unconjugated
Cellular Localization:Cell junction, Cell membrane, Cytoplasm, Cytoplasmic side, Nucleus, Peripheral membrane protein, cell cortex, centrosome, cytoskeleton, focal adhesion, microtubule organizing center.
Calculated MW:119kDa
Observed MW:125kDa

This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.

Purification Method:Affinity purification
Gene ID:5747
Clone Number:ARC62271
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,0.05% BSA,50% glycerol,pH7.3.
Product ImageWestern blot analysis of A-549, using Phospho-FAK-Y397 Rabbit mAb (CABP1447) at 1:4000 dilution.A-549 cells were treated by λ-PP mixed solution (1ul) at 30℃ for 30 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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