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Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 Polyclonal Antibody (CABP1284)

SKU:
CABP1284
Product Type:
Antibody
Antibody Type:
Monoclonal Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
$215 - $563
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 Polyclonal Antibody
SKU:CABP1284
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around T567 of human Ezrin/Radixin/MoesinEZR (NP_003370.2).
Sequence:YKTL R
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:1000
Synonyms:CVL; CVIL; VIL2; HEL-S-105; DFNB24; HEL70; IMD50; Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558
Positive Sample:HeLa,A-431
Conjugate:Unconjugated
Cellular Localization:actin cytoskeleton, apical plasma membrane, basolateral plasma membrane, cell projection, ciliary basal body, cortical cytoskeleton, cytoplasm, cytoplasmic side of apical plasma membrane, cytosol, endosome, extracellular exosome, extracellular space, fibrillar center, filopodium, focal adhesion, immunological synapse, microvillus membrane, perinuclear region of cytoplasm, plasma membrane, plasma membrane raft, ruffle membrane
Calculated MW:69kDa/68kDa/67kDa
Observed MW:75kDa/80kDa

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.

Purification Method:Affinity purification
Gene ID:7430/5962/4478
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 antibody (CABP1284) at 1:1000 dilution.HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

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