The Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody (CABP1284) is a vital tool for researchers interested in studying the phosphorylation of the ezrin-radixin-moesin (ERM) protein family. This antibody, produced using advanced technology, specifically targets the phosphorylated forms of ezrin at Thr567, radixin at Thr564, and moesin at Thr558.The ERM proteins play essential roles in cell structure and function, including cell adhesion, migration, and membrane-cytoskeleton interactions. Phosphorylation of these proteins at the specified sites is known to modulate their activity and influence cellular processes. The CABP1284 antibody enables the detection and analysis of phosphorylated ERM proteins in various cell types, providing valuable insights into their function and regulation.
Researchers can use this antibody in Western blot applications to investigate the phosphorylation status of ezrin, radixin, and moesin in different experimental conditions. The antibody's high specificity and sensitivity ensure reliable results, making it a versatile tool for studies in cell biology, cancer research, and other fields where ERM protein phosphorylation is of interest. Overall, the Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody (CABP1284) is a valuable resource for scientists seeking to understand the role of ERM protein phosphorylation in
actin cytoskeleton, apical plasma membrane, basolateral plasma membrane, cell projection, ciliary basal body, cortical cytoskeleton, cytoplasm, cytoplasmic side of apical plasma membrane, cytosol, endosome, extracellular exosome, extracellular space, fibrillar center, filopodium, focal adhesion, immunological synapse, microvillus membrane, perinuclear region of cytoplasm, plasma membrane, plasma membrane raft, ruffle membrane
Calculated MW:
69kDa/68kDa/67kDa
Observed MW:
75kDa/80kDa
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene.Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement.
Purification Method:
Affinity purification
Gene ID:
7430/5962/4478
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Phospho-Ezrin-Thr567/Radixin-Thr564/Moesin-Thr558 antibody (CABP1284) at 1:1000 dilution.HeLa cells were treated by TNF-α (20 ng/ml) and Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
