The Phospho-EZR (T567)/RDX (T564)/MSN (T558) Rabbit Polyclonal Antibody (CABP1121) is a valuable tool for researchers studying phosphorylation events in key proteins involved in cell motility and signaling pathways. This antibody is raised in rabbits and has high reactivity with human samples, making it ideal for Western blot applications.Phosphorylation of EZR, RDX, and MSN is known to be involved in regulating processes such as cell migration, adhesion, and cytoskeletal organization. This antibody specifically targets the phosphorylated forms of these proteins, allowing for the detection and analysis of their activity in various cell types.
By understanding the phosphorylation status of EZR, RDX, and MSN, researchers can gain insights into the molecular mechanisms underlying cell motility and signaling, which are crucial for studies in cancer biology, developmental biology, and cell biology. This antibody is a valuable tool for investigating the role of these proteins in physiological and pathological processes, and for identifying potential therapeutic targets in diseases such as cancer and metastasis.
A synthetic phosphorylated peptide around T567 of human EZR/RDX/MSNEZR (NP_003370.2).
Sequence:
YKTL R
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Positive Sample:
PC-12
Observed MW:
80kDa
The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene.
Purification Method:
Affinity purification
Gene ID:
7430/4478/5962
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from PC-12 cells, using Phospho-EZR-T567/RDX-T564/MSN-T558 Rabbit pAb (CABP1121) at 1:1000 dilution.PC-12 cells were treated by ATP(5 mM) at 30℃ for 1 hour.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
