The Phospho-EZH2 (KMT6) T311 Antibody (CABP1269) is a valuable tool for researchers studying the activity of EZH2, a key player in epigenetic regulation and cancer progression. This antibody, produced in rabbits, specifically targets the phosphorylated form of EZH2 at threonine 311, allowing for precise detection and analysis in human samples.EZH2, a member of the Polycomb group proteins, is known for its role in gene repression through histone methylation. The phosphorylation of EZH2 at T311 has been linked to its oncogenic function in various cancer types, making it a crucial biomarker for assessing disease progression and potential therapeutic targets.
With its high reactivity and specificity, the Phospho-EZH2 (KMT6) T311 Antibody is validated for use in Western blot applications, providing researchers with a reliable tool for investigating the role of EZH2 phosphorylation in cancer development and progression. Its potential applications extend to studies of epigenetic regulation, chromatin remodeling, and the development of targeted therapies for cancer treatment.
Product Name:
Phospho-EZH2/KMT6-T311 Polyclonal Antibody
SKU:
CABP1269
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Rat
Immunogen:
A synthetic phosphorylated peptide around T311 of human EZH2/KMT6 (NP_001190176.1).
This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene.
Purification Method:
Affinity purification
Gene ID:
2146
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of C6 cells, using Phospho-EZH2/KMT6-T311 antibody (CABP1269) at 1:1000 dilution.C6 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
