The Phospho-Estrogen Receptor S118 Polyclonal Antibody (CABP0146) is a valuable tool for researchers studying estrogen receptor signaling pathways. This antibody, raised in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the phosphorylated form of the Estrogen Receptor at Serine 118, this antibody enables researchers to detect and analyze the activation of this critical signaling molecule in various cell types.The Estrogen Receptor is a nuclear receptor that plays a key role in regulating gene expression in response to estrogen. Phosphorylation of the Estrogen Receptor at Serine 118 is known to enhance its transcriptional activity, influencing the expression of genes involved in cell proliferation, differentiation, and survival.
Understanding the phosphorylation status of the Estrogen Receptor is essential for unraveling the complex mechanisms of estrogen signaling in normal physiology and diseases like breast cancer.By using the Phospho-Estrogen Receptor S118 Polyclonal Antibody, researchers can gain valuable insights into the activation and regulation of the Estrogen Receptor in cellular processes. This antibody is a valuable tool for studies in cancer biology, endocrinology, and hormone-related disorders, paving the way for the development of targeted therapies and personalized treatments in the future.
Product Name:
Phospho-ESRα-S118 Rabbit Polyclonal Antibody
SKU:
CABP0146
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1) (NP_000116.2).
Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
Calculated MW:
66kDa
Observed MW:
66kDa
This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.
Purification Method:
Affinity purification
Gene ID:
2099
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of MCF7 cells, using Phospho-ESRα-S118 pAb (CABP0146) at 1:1000 dilution.MCF7 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
