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Phospho-ESRα-S118 Rabbit Polyclonal Antibody (CABP0146)

SKU:
CABP0146
Product Type:
Antibody
Applications:
WB
IHC
IF
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-Phospho-Estrogen receptor-S118 Antibody CABP0146
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-Phospho-Estrogen receptor-S118 Antibody CABP0146
  • Epigenetics and Nuclear Signaling Antibodies 4 Anti-Phospho-Estrogen receptor-S118 Antibody CABP0146
€419
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Description

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Product Name:Phospho-ESRα-S118 Rabbit Polyclonal Antibody
SKU:CABP0146
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human
Immunogen:A synthetic phosphorylated peptide around S118 of human Estrogen Receptor alpha (ESR1) (NP_000116.2).
Sequence:QLSP F
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:WB,1:500 - 1:2000 IF/ICC,1:100 - 1:200
Synonyms:ER; ESR; Era; ESRA; ESTRR; NR3A1; Phospho-ESRα-S118
Positive Sample:MCF7
Conjugate:Unconjugated
Cellular Localization:Cell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein.
Calculated MW:66kDa
Observed MW:66kDa

This gene encodes an estrogen receptor and ligand-activated transcription factor. The canonical protein contains an N-terminal ligand-independent transactivation domain, a central DNA binding domain, a hinge domain, and a C-terminal ligand-dependent transactivation domain. The protein localizes to the nucleus where it may form either a homodimer or a heterodimer with estrogen receptor 2. The protein encoded by this gene regulates the transcription of many estrogen-inducible genes that play a role in growth, metabolism, sexual development, gestation, and other reproductive functions and is expressed in many non-reproductive tissues. The receptor encoded by this gene plays a key role in breast cancer, endometrial cancer, and osteoporosis. This gene is reported to have dozens of transcript variants due to the use of alternate promoters and alternative splicing, however, the full-length nature of many of these variants remain uncertain.

Purification Method:Affinity purification
Gene ID:2099
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of MCF7 cells, using Phospho-ESRα-S118 pAb (CABP0146) at 1:1000 dilution.MCF7 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.

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